WebEngineering of designer homing endonucleases has set the stage for genome editing of complex eukaryotic genomes with a broad range of potential applications including targeted gene knockouts in model organisms and gene therapy in humans, making this book a valuable resource for future research.

Homing endonucleases: structure, function and evolution

Web17 sep. 2024 · Germline homing and transmission of naturally occurring and engineered HEGs into artificial target sites, and of engineered HEGs into endogenous sites, have been demonstrated recently in mosquitoes and Drosophila (7, 12–22).In particular, a number of recent experiments have demonstrated significant rates of germline homing using HEGs … WebI-CreI is a homing endonuclease whose gene was first discovered in the chloroplast genome of Chlamydomonas reinhardtii, a species of unicellular green algae. [1] It is named for the facts that: it resides in an I ntron; it was isolated from Clamydomonas reinhardtii; it was the first ( I) such gene isolated from C. reinhardtii. bridgecrest ga

Homing endonucleases: keeping the house in order

WebHoming endonucleases are microbial DNA-cleaving enzymes that mobilize their own reading frames by generating double strand breaks at specific genomic invasion sites. … Web10 apr. 2007 · Homing endonucleases are a distinctive class of site-specific DNA endonucleases that promote the lateral transfer of their own coding region and flanking … Web1 sep. 1997 · Homing endonucleases are rare-cutting enzymes encoded by introns and inteins. They have striking structural and functional properties that distinguish them from … bridgecrest gps