Hct116 puromycin
WebApr 12, 2024 · Puromycin was added to the cells for 5 days and individual cell colonies were isolated. The knockout effect was examined by Western blot with DNMT1 antibody (clone EPR3521(2); Abcam) in the ... WebAug 29, 2002 · A colorectal carcinoma cell line, HCT116, which has both mutated and wild-type β-catenin genes, was engineered by homologous recombination to investigate the significance of β-catenin gene mutation.
Hct116 puromycin
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WebHCT116 cells have been widely used in colorectal cancer research. HCT116/GFP (CMV) stable cells express GFP under the control of the CMV promoter. ... Puromycin: Biosafety Level: BSL-2: Shipped: Dry ice : Storage: Liquid Nitrogen: Catalog Number Product Size Price Qty; SC-1001. HCT 116/GFP (CMV) stable cells. 2x10^6 cells: Price: Quantity. … WebPuromycin is an aminonucleoside antibiotic produced by Streptomyces alboniger. It specifically inhibits peptidyl transfer on both prokaryotic and eukaryotic ribosomes. This …
WebJan 1, 2016 · Homeowners aggrieved by their homeowners associations (HOAs) often quickly notice when the Board of Directors of the HOA fails to follow its own rules, or … WebDec 30, 2024 · Puromycin is used for selection and maintenance of cell lines expressing a transfected pac gene (S. alboniger), whose product, puromycin acetyltransferase, inactivates puromycin via acetylation; recommended concentration in cell culture 1-10µg/ml. Lot-to-lot variations in potency exist for all selection antibiotics, each new lot of …
WebDual-labeled HCT116 cell line expressing GFP at 90% cell confluence. Panel B: Dose-response luciferase activity analyses in HCT116 cell line after Staurospine treatment. … WebFeb 22, 2010 · For IL4Ra knockdown in HCT116 cells, ‘Sure-Silencing’ plasmids encoding shRNA sequences directed toward four different regions of human IL4Ra ... murine or human cells were placed under selection with puromycin (10 μg/ml) or hygromycin (200 μg/ml), respectively. Once stable populations were obtained, individual clones were …
WebGibco® fetal bovine serum, puromycin, ... HCT116 and HT29 cells were cultured in growth medium (DMEM supplemented with 10% heat-inactivated fetal bovine serum, 1% penicillin/streptomycin) in a humidified atmosphere with 5% CO2 at 37 °C. Both malignant cell lines were stably transfected with shSep15 or a control construct
WebSPOLIATION OF EVIDENCE From the Georgia Bar Journal By Lee Wallace The Wallace Law Firm, L.L.C. 2170 Defoor Hills Rd. Atlanta, Georgia 30318 404-814-0465 buck\u0027s automotive tucsonWebApr 1, 2024 · Stable transfection of HCT116 p53 +/+ and HCT116 p53 −/− cell lines (a kind gift from Dr. B. Vogelstein) were performed as described previously [17], Transfected cells were selected by culturing in medium containing 4 μg/ml of puromycin (Sigma-Aldrich), and single cell colonies were isolated. Immunoblots buck\u0027s bar venice nebraskaWebNational Center for Biotechnology Information buck\u0027s bar veniceWebMar 11, 2016 · Most recent answer 11th Dec, 2024 Ching Yi Ong Universiti Sains Malaysia Hi, I am working on HCT116 and HT29 cells. May I know what was the concentration of … buck\u0027s bbqWebUsage Information. HCT116-eGFP-Puro is a polyclonal population of the human colorectal carcinoma cell line HCT116 (ATCC® CCL-247™). To achieve stable reporter expression in the polyclonal population, parental … buck\\u0027s bar venice nebraskaWebApr 12, 2024 · The maximal tumor growth delays in HCT116- DNMT1+/+ model were observed at day 12 and day 18 by decitabine and aza-T-dCyd treatment, respectively. … buck\\u0027s bbqWebOct 28, 2013 · HCT116 TCF4/Wnt cells were cultured in the presence of 50 μg ml −1 hygromycin B, and HCT116 TCF/LEF cells were cultured in the presence of 10 μg ml −1 puromycin. Primary colon cancer ... buck\u0027n good donuts seymour