WebFeb 14, 2024 · ChIP followed by tiling microarray hybridization (ChIP-chip) or high throughput sequencing (ChIP-seq) are widely used for studying genome-wide protein-DNA interactions. In previous work, we applied ChIP-chip to T. gondii and showed that the distributions of epigenomic modifications on chromatin are conserved in protozoa [ 22 ]. WebDec 17, 2011 · The feasibility of γ-H2AX ChIP-seq analysis in this work indicates that corresponding chromatin is accessible for nucleases in mitotic chromosomes, which are notoriously refractory to ChIP approach for many other chromatin proteins.
Histone H2A.X antibody (pAb) (H2AX) - Active Motif
Web(a) INDUCE-seq detects both highly recurrent induced DSBs and low-level endogenous DSBs simultaneously with high resolution. Genome browser view (IGV) of INDUCE-seq reads mapped to a 10Mb section of the genome from HEK293T cells following in situ cleavage with the restriction endonuclease HindIII. WebJan 20, 2024 · Once significant human genome sequencing began for the HGP, a 'draft' human genome sequence (as described above) was produced over a 15-month period … task book evaluation
(PDF) Profiling DNA break sites and transcriptional
WebWe combined proteomic, ChIP-seq, and transcriptome data to show that HMGA2-induced transcription requires phosphorylation of the histone variant H2AX at S139 (H2AXS139ph; γ-H2AX) mediated by the protein kinase ataxia telangiectasia mutated (ATM). WebJan 4, 2024 · (D) Browser view of macroH2A1.2 and γ-H2AX ChIP-seq data within the FRA1I CFS. Data are log2 ChIP/Input, and tracks are displayed on scales from 0 to 1.8. (E) Overlap of macroH2A1.2 peaks with various repeat classes, when overlapping γ-H2AX (γ-H2AX +) or not (γ-H2AX −). A randomly shuffled set of peaks is presented as a control. WebJun 9, 2024 · NAIL-seq identifies early DNA replication initiation zones. To identify replication initiation zones with the aim of tracing early DNA replication, we synchronized … task-based assessment