WebThe intensities of the fluorescence peaks for CD63-mCherry showed that it is localized predominantly at the donor cell site. However, after transfer of HIV-1 JRFL Gag-iGFP … WebWir bieten eine breite Palette validierter antikrper zur Verwendung als Lysosome Markers. ️ Hohe Qualität ️ Niedrige Preise ️ 100% Garantie
Time-lapse imaging of CD63 dynamics at the HIV-1 virological …
WebTo test whether the introduction of GFP-type termini into mRFP variants would benefit fusion proteins expressed in mammalian cells, we fused mRFP1 and mCherry to the N … WebCD63–mCherry was a gift from Dr. Mark McNiven, and CD63–GFP was from Addgene. We are also grateful to Dr. David Katzmann and Dr. Brian Davies for useful discussions and insight into MVB formation, and Courtney Hoover for outstanding administrative support. The optical microscopy core of the Mayo Clinic Center for Cell Signaling was ... mongo use the force argument to override
mCherry :: Fluorescent Protein Database
WebER50BleoR selected for the highest level of mCherry expression, greater than twofold higher than BleoR or any other AR gene. Interestingly, use of ER50BleoR as the selectable marker translated to an even higher, 3.5-fold increase in the exosomal loading of the exosomal cargo protein, CD63/Y235A. WebU87 MG cells transfected with a CD63-mCherry plasmid were sorted with FACSAria by fluorescence intensity and clones were further selected by neomycin resistance. U87 MG-CD63-mCherry cells were routine cultured in growth medium supplemented with 1000 μg/ml G418. All cells were cultured in a humidified incubator with 5% CO 2 at 37 °C. WebMar 10, 2024 · separately transfected with the CD63-mCherry, CD63-phiYFP, CD63-eGFP and CD63-mTFP plasmids to obtain stable clones expressing only one of the CD63-XFP … mongo user 一覧