Camyel assay
WebMeanwhile, PCR positive results (15%, 15%, and 20%) in male, Non-LF, and LF blood samples collected from camels were similar to those obtained using the LAMP assay when examining the same samples True positive Sensitivity = True positive+False negative × 100 Sensitivity LAMP assay on blood samples = 22/22 + 0 * 100 = 100% Sensitivity LAMP ... WebThe assay utilises a commonly used cAMP bioluminescence resonance energy transfer (BRET) biosensor to detect changes in cAMP levels in live cells. This method was used …
Camyel assay
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WebA retroviral vector for introducing the CAMYEL (cAMP sensor using YFP-Epac-RLuc) into mammalian cells. It has been used to facilitate studies of cAMP regulation using BRET (bioluminescence resonance energy transfer) by quantitatively and rapidly monitoring intracellular concentration of cAMP in vivo. Vector features: 5'LTR: 209-760; viral … Webprocessing and analysis tools deliver a large assay window and improved sensitivity over methods relying on total fluorescence intensity measurements. Dual in-line dispense tips and rapid image capture rates support sequential addition of Gs- and Gi-coupled receptor agonists for detailed characterization of cAMP-dependent signaling pathways.
WebThe CAMYEL assay can thus iden- tify novel inhibitors of Epac1, whose site of action is not in the cAMP-binding site but likely in the hinge region of the protein. FIGURE 11. Molecular model of ... WebCAMYEL protein was expressed in bacteria and purified for further characterization. A concentration response analysis with cAMP indicated a K d and n (Hill coefficient) for binding of 8.8 μM and ...
WebSep 11, 2024 · CAMYEL assays. β 2 -adrenoceptor +/- 10 μM salbutamol was used as a positive control for constitutive cAMP signaling. Data represent n=3-4 assays performed in triplicate and final ligand concentrations were 10 μM. (c) CAMYEL assay performed as in (b) using CHO-K1 cells to control for cellular background; n=3 performed in triplicate. … WebJul 12, 2024 · For the cAMP assay, HEK293 cells were transiently transfected with either GIPR wt or GIPR-[E354Q] and the bioluminescence resonance energy transfer (BRET) Epac-based sensor for cAMP, CAMYEL (cAMP sensor using YFP-Epac-RLuc). 33 For the β-arrestin 2 assay, HEK293 cells were transiently transfected with either GIPR wt or …
WebJun 22, 2024 · a Conceptual representation of the MOR CAMYEL BRET assay. To measure G protein activation, MOR (light green) was coexpressed with G protein subunits G α oB …
WebRead how the CAMYEL BRET biosensor can be used to study the associated signaling pathways in plate readers. ... Assay Principle. The CAYMEL BRET biosensor 3 is composed of the EPAC protein bound to both a luciferase (RLuc) and yellow fluorescent protein (YFP) as shown in fig. 1. In the absence of cAMP, the conformation of EPAC … thompson howle seattleWebMar 1, 2024 · c, CAMYEL assay performed as in b using CHO-K1 cells to control for cellular background. Data represent n = 3 biological replicates. AR, adrenoceptor. uk to kolkata direct flightWebSep 24, 2024 · CAMYEL detected concentration-dependent changes in cAMP activity at RXFP1-4 in recombinant cell lines, using a variety of ligands with potencies comparable to those seen in conventional cAMP … thompson hr formsWebJul 15, 2014 · ( b) The mouse MOR was co-expressed with Gα oB, β 1, γ2 and the bioluminescence resonance energy transfer (BRET) sensor CAMYEL to assay inhibition of forskolin-stimulated cAMP accumulation. thompson howellWebSep 26, 2024 · The assay is based on bioluminescence resonance energy transfer (BRET) that involves transfection of a biosensor in human embryonic kidney (HEK)-293 cells … thompson howle vaughn seattleWebIn this work, the camel cytochrome P450 2E1 (CYP2E1) is compromised to detect its evolution rate and its power to bind with various chemicals, protoxins, procarcinogens, … thompson howle vaughn attorneys at law llpWebSep 24, 2024 · CAMYEL detected concentration-dependent changes in cAMP activity at RXFP1-4 in recombinant cell lines, using a variety of ligands with potencies comparable … uk to kochi flight price